Journal: International Journal of Nanomedicine

Article Title: Ginseng-berry-mediated gold and silver nanoparticle synthesis and evaluation of their in vitro antioxidant, antimicrobial, and cytotoxicity effects on human dermal fibroblast and murine melanoma skin cell lines

doi: 10.2147/IJN.S118373

Figure Lengend Snippet: Comparative study of the effect of GBAuNPs, GBE and HAuCl 4 ·3H 2 O on cell viability and on the morphology of HDF and B16 cells. Notes: Optical microscopy images ( A ) of HDF and B16 cell lines (40× magnification) at 72 h after treated with 1–100 μg/mL of GBAuNPs. Treated HDF and B16 cells with AuNPs (10 and 100 μg/mL) could be identified by dark dense clusters which are indicated with arrows; no cluster was found in control groups. Cytotoxicity effect of GBE and GBAuNPs on HDF ( B ) and B16BL6 ( C ) cell lines; GBAuNPs did not exhibit a significant cytotoxic effect on HDF and B16 cells. Cell viability was determined by MTT assay. Data are expressed as a percentage of sample-treated control and presented as mean ± SEM of three separate experiments. B16, murine melanoma B16BL6 cells. Abbreviations: AuNPs, gold nanoparticles; GBAuNPs, AuNPs from ginseng berry; GBE, ginseng berry extract; HDF, human dermal fibroblast; SEM, standard error of the mean.

Article Snippet: HDF and B16 cell lines were obtained from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Microscopy, Control, MTT Assay

Journal: International Journal of Nanomedicine

Article Title: Ginseng-berry-mediated gold and silver nanoparticle synthesis and evaluation of their in vitro antioxidant, antimicrobial, and cytotoxicity effects on human dermal fibroblast and murine melanoma skin cell lines

doi: 10.2147/IJN.S118373

Figure Lengend Snippet: Comparative study of the effect of GBAgNPs, GBE and AgNO 3 on cell viability and on the morphology of HDF and B16 cells. Notes: Optical microscopy images ( A ) of HDF and B16 cell lines (40× magnification) at 72 h after treated with 1–100 μg/mL of GBAgNPs. Treated HDF and B16 cells with silver (10 μg/mL) could be identified by dark dense clusters which are indicated with arrows; no cluster was found in control groups. Cytotoxicity effect of GBE, GBAgNPs, and silver salts on HDF ( B ) and B16 ( C ) cell lines; cell viability decreased with an increase in the concentration of GBAgNPs. Cell viability was determined by MTT assay. Data are expressed as a percentage of sample-treated control and presented as mean ± SEM of three separate experiments. Abbreviations: B16, murine melanoma B16Bl6 cells; GBAgNPs, silver nanoparticles from ginseng berry; GBE, ginseng berry extract; HDF, human dermal fibroblast; MTT, 3-(4,5-dimethyl-thiazol-2yl)-2, 5-diphenyl tetrazolium bromide; SEM, standard error of the mean.

Article Snippet: HDF and B16 cell lines were obtained from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Microscopy, Control, Concentration Assay, MTT Assay

Journal: RSC Advances

Article Title: In vitro and in vivo biocompatibility and inflammation response of methacrylated and maleated hyaluronic acid for wound healing

doi: 10.1039/d0ra06025a

Figure Lengend Snippet: Cell proliferation investigation of HUVEC (a), HDF (b) and HAD-MSC (c) seeded on MEHA and MAHA at long time (1, 3 and 7 days). Cell proliferation was performed by CCK8 assay using manufacturer's protocol. *, p < 0.05; **, p < 0.01; ***, p < 0.0001; ns, no significant difference.

Article Snippet: HDF, HAD-MSC and HUVEC (ScienCell – California, USA) were cultured in 75 cm 2 cell culture flask.

Techniques: CCK-8 Assay

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Assessment of chemotherapeutic effects on cancer cells using adhesion noise spectroscopy

doi: 10.3389/fbioe.2024.1385730

Figure Lengend Snippet: 5-Fluorouracil (5-FU) with cytotoxic and detachment-enhancing effect on the colorectal cancer cell (CRC) line HT-29 but without effect on human dermal fibroblast (HDF) cell viability and proliferation. (A) 5-FU effects via CAN spectroscopy on HT-29 cells cultivated on the CMOS MEA (analyzed at 300 kHz). Untreated HT-29 grew on the CMOS MEA and the cell-covered area rose (A-i) . After 5-FU treatment, the detection positive area declined after 72 h of cultivation (A-ii) . Statistics (A-i,A-ii) : one-way ANOVA. Statistical difference of the cell-covered area between untreated and treated HT-29 (A-iii) . Statistics (A-iii) : two-way ANOVA. (B) 5-FU effects via CAN spectroscopy on HDF cells cultivated on the CMOS MEA (analyzed at 300 kHz). Untreated and treated HDFs showed a stagnant CMOS MEA area where cells are adhered to (B-i,B-ii) . Statistics (B-i,B-ii) : one-way ANOVA. CMOS MEA area covered by HDFs (B-iii) . Statistics (B-iii) : two-way ANOVA. (C) 5-FU effects on HT-29 cells via CCK-8 assay. Untreated HT-29 cells’ optical density (OD) increased during 72 h cultivation time (C-i) , while 5-FU treated cells’ OD declined (C-ii) . (D) 5-FU effects on HDF cells via CCK-8 assay. For HDFs, the OD stays constant without (D-i) and with 5-FU treatment (D-ii) . Statistics: one-way ANOVA. (C-iii,D-iii) compared cell viability of treated cells with untreated cells as control group. Treated cells showed less cell viability for both cell types [HT-29 (C-iii) and HDF (D-iii) ]. Statistics: two-way ANOVA. (E) Cell viability from CASY recordings of treated and untreated HT-29 in the supernatant and on the chip (E-i) . Same for HDFs (E-ii) . Two-way ANOVA. Statistical significance is indicated by asterisks (n HT-29 = 4, n HDF = 4, ns, not significant. *, p ≤ 0.05. **, p ≤ 0.01. ***, p ≤ 0.001. ****, p ≤ 0.0001).

Article Snippet: The colorectal cancer (CRC) cell line HT-29 (ATCC, RRID: CVCL_0320) and the human dermal fibroblast cell line HDF (PELOBiotech GmbH, RRID: CVCL_DP66) were cultivated at 37°C in a 5% CO 2 atmosphere.

Techniques: Spectroscopy, CCK-8 Assay, Control